cyclic AMP Complete EIA Kit

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Description Size Catalog# Price Quantity  
cyclic AMP Complete EIA Kit   1x96 well 900-163 $350.00  
cyclic AMP Complete EIA Kit   5x96 well 901-163 $1,450.00  
Background
The Assay Designs cAMP Complete ELISA is a flexible assay to measure either intra- or extracellular cAMP in a wide variety of sample matrices. An acid solution is provided to prepare cell and tissue samples to stop endogenous phosphodiesterase activity. Aceltylation reagents are also provided to offer an ~10-fold increase in assay sensitivity.

Cyclic AMP (cAMP) is one of the most important second messengers involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

Assay Designs also provides cyclic AMP EIA kits in a convenient 384 well format upon request. Please contact info@assaydesigns.com for additional information.

Table of Abbreviations

Applications
EIA

Species Reactivity
Species Independent

Features

  • Versatile - one convenient ELISA to assay both intracellular and extracellular cAMP
  • Simple - easy-to-use liquid color-coded reagents reduce errors
  • Sensitive - measure as little as 0.037 pmol/mL cAMP

Range
0.78 - 200 pmol/mL (non-acetylated); 0.078 - 20 pmol/mL (acetylated)

Cross Reactivities
cAMP 100%, AMP <0.33%, ATP <0.12%, cGMP, GMP, GTP, cUMP, CTP <0.001%

Sample Types
CL, CS, S, SA, T

Samples Per Kit
39 samples in duplicate per 96 wells

Sensitivity
Assay Buffer : 0.30 pmol/mL (non-acetylated) 0.039 pmol/mL (acetylated) HCl: 0.39 pmol/mL (non-acetylated) 0.037 pmol/mL (acetylated)

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout

Product Manual

Product Name Catalog # Manual
cyclic AMP Complete EIA Kit 900-163   Kit Insert     MSDS    
cyclic AMP Complete EIA Kit 901-163   Kit Insert     MSDS    

Ordering Info
Click here to learn more about online ordering
Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134