Grp94 Monoclonal Antibody (9G10), DyLight 488

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
Grp94 Monoclonal Antibody (9G10), DyLight 488 Conjugate  50 µg SPA-850-488D $185.00  
Grp94 Monoclonal Antibody (9G10), DyLight 488 Conjugate  200 µg SPA-850-488F $365.00  
Background
Glucose-regulated protein 94 (Grp94, gp96), an abundant resident endoplasmic reticulum (ER) lumenal stress protein, belongs to the Hsp90 family of molecular chaperones along with cytosolic Hsp90. Grp94 and such other resident soluble proteins of the ER as the Ca 2+ binding protein subfamily (CaBP, CaBPI, CaBP2 and calreticulin) possess the C-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL), a sorting signal considered responsible for the retention of these proteins in the pre-Golgi compartments. Stress conditions such as glucose starvation and heat shock which promote protein misfolding or unfolding increase Grp94 expression. In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. Grp94 and Hsp90 share high sequence identity and apparently identical adenosine nucleotide dependent modes of regulation, although previous data suggests that Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the binding site for the Hsp90 inhibitors geldanamycin and radicicol. However, the molecular basis for adenosine nucleotide-dependent regulation of Grp94 remains unclear. Data supports a ligand dependent regulation of Grp94 function, and suggests a model whereby Grp94 function is regulated through a ligand -dependent conversion of Grp94 from an inactive to an active conformation.

Table of Abbreviations

Abbreviated Name
Grp94 mAb, DyLight 488 (9G10)

Conjugate
DyLight 488

Applications
Flow

Isotype
IgG2a

Purification
Protein G Affinity

Immunogen
Native chicken Grp94 protein

Host
Rat

Clone Number
9G10

Product Image(s): click to enlarge

Source
Rat mAb

Species Reactivity
H, M, R, B, C, CH, HA, GP, MO, RB, P,S, X

For research use only, not for diagnostic or therapeutic use.

Documentation
MSDS
Spec Sheet

Product Manual

Product Name Catalog # Manual
Grp94 Monoclonal Antibody (9G10), DyLight 488 Conjugate SPA-850-488D  
Grp94 Monoclonal Antibody (9G10), DyLight 488 Conjugate SPA-850-488F  

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Product Name Catalog#
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Grp94 Polyclonal Antibody
 		SPA-851
Grp94 Recombinant Canine Protein
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IgG2a Isotype Control Monoclonal Antibody (C1.18), DyLight 488
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Citations
Launch Citation Database

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