HO-1 (Hsp32) Monoclonal Antibody (HO-1-2), PE Conjugate

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
HO-1 (Hsp32) Monoclonal Antibody (HO-1-2), PE Conjugate  50 µg OSA-111PED $235.00  
HO-1 (Hsp32) Monoclonal Antibody (HO-1-2), PE Conjugate  200 µg OSA-111PEF $395.00  
Background
Heme oxygenase-1 (HO-1) or HSP32 is the inducible isoform of heme oxygenase which catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, iron and biliverdin that is immediately reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'-untranslated regions (UTRs) of the mRNAs. To date, three heme oxygenase isoforms HO-1, HO-2 and HO-3 have been identified. HO -1, also known as Hsp32, a major heat shock/stress response protein, is ubiquitous and its mRNA as well as its activity can be increased several-fold by heme, other metalloporphyrins, transition metals and stimuli that induce cellular stress. The 5'- UTR of HO-1 has several consensus regulatory elements which include sites for activator protein 1 (AP-1), metal responsive element (MRE), oncogene c-myc/max heterodimer binding site (Myc/Max), antioxidant response element (ARE) and GC box binding (Sp1). HO-1 expression has been shown to increase in benign prostatic hyperplasia (BPH) and malignant prostate tissue suggesting a role for this stress protein in the pathogenesis of BPH and prostate cancer. There is recent data which indicates the ability of peroxynitrite (ONOO-) to modulate the expression of HO-1 and suggest that the heme oxygenase pathway contributes to protection against the cytotoxic action of ONOO- which is a potent oxidizing agent generated by the interaction of nitric oxide (NO) and the superoxide anion. ONOO- rapidly decomposes to a highly reactive hydroxyl radical and nitrogen dioxide, both of which cause oxidative damage.

Table of Abbreviations

Product Image(s): click to enlarge

Source
Mouse mAb

Immunogen
Native rat HO-1 protein

Purification
Protein G Affinity

Applications
Flow

Species Reactivity
H, M, R, C, GP, H, MO

For research use only, not for diagnostic or therapeutic use.

Documentation
MSDS
OSA-111PE Cert. of Analysis Lot 03030822
Spec Sheet

Product Manual

Product Name Catalog # Manual
HO-1 (Hsp32) Monoclonal Antibody (HO-1-2), PE Conjugate OSA-111PED  
HO-1 (Hsp32) Monoclonal Antibody (HO-1-2), PE Conjugate OSA-111PEF  

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Citations
Launch Citation Database

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