Hsp70 High Sensitivity ELISA Kit

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
Hsp70 High Sensitivity ELISA Kit 1x96 well  EKS-715 $575.00
Background
Inducible heat shock protein 70 (Hsp70, or Hsp72) is a stress protein whose expression is upregulated when the cell or organism is placed under conditions of stress. Hsp70 is essential for cellular recovery, survival and maintenance of normal cellular function. As a molecular chaperone, Hsp70 prevents protein aggregation and refolds damaged proteins in response to cellular stress caused by environmental insults, pathogens and disease. Hsp70 is also released into the circulation from both necrotic and non-necrotic cells, and its levels in serum are increased by a number of stressors. Hsp70 is believed to serve as an immunological "danger signal," and measurement of Hsp70 in serum and plasma is a useful tool in the study of heat stress, infection, cardiovascular disease, exercise, and psychological stress.

Applications
EIA

Features

  • Ultra-sensitive ELISA for the measurement of Hsp70 in serum and EDTA plasma samples
  • Fast results in just 4.5 hours
  • Highly specific for inducible Hsp70 (Hsp72)

Range
0.20 - 12.5 ng/mL

Cross Reactivities
"Hsp72 (human) 100% ... Hsp70 (rat) 117.6% / Hsp70 (mouse from A2 gene, cytosolic) 33.6% / Hsp70 (salmon) 0.8% / Hsp70Bż (human) 5.4% / DnaK (E.coli) 0.5% / Hsc70 (Bovine) <0.016% / Grp78 (hamster) <0.016% / Hsp71 (M. tuberculosis) <0.016%

Sample Types
serum, EDTA plasma

Samples Per Kit
39 in duplicate

Sensitivity
90 pg/mL

Significance and Uses
Assay Designs Hsp70 High Sensitivity EIA Kit is a fast and sensitive method to easily analyze serum and plasma samples from human, mouse, and rat origins. This assay allows for the specific determination of both native and recombinant Hsp70 (Hsp72). It does not detect other Hsp70 family members such as Hsc70 (Hsp73), Grp78, DnaK (E. coli), or Hsp71 (M. tuberculosis).

Species Reactivity
H, M, R

Time To Answer
4.5 hours

For research use only, not for diagnostic or therapeutic use.

Ordering Info
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