Hsp27 Monoclonal Antibody (G3.1), R-Phycoerythrin Conjugate

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
Hsp27 Monoclonal Antibody (G3.1), R-Phycoerythrin Conjugate  100 µg SPA-800PEE $325.00  
Background
Human Hsp27, mouse Hsp25 and alpha B crystallin belong to a diverse family of small heat shock proteins produced in all organisms. Chaperone-like proteins which bind unfolded polypeptides and prevent uncontrolled protein aggregation, Hsp27 proteins appear to function mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells, and data suggests that the large oligomers of Hsp27 act as chaperones by providing a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. The state of phosphorylation and oligomerization of Hsp27 may regulate microfilament organization in light of data showing only the nonphosphorylated lower molecular weight forms of Hsp27 binding actin barbed ends and inhibiting polymerization. Hsp27 also appears to protect cells by enhancing cellular glutathione levels; cells overexpressing Hsp27 incur elevated glutathione levels. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates reveal that cellular glutathione levels depend on the oligomerization of Hsp27. Recent data suggests a novel function for Hsp27 as a negative regulator of cytochrome c-dependent activation of procaspase-3.

CloneNumber
G3.1

Applications
Flow

Species Reactivity
H, MO

Host Species
Mouse

Format
PBS, pH 7.2, 0.09% azide

Purification Method
Protein G Affinity

Immunogen
Native human Hsp27 protein

For research use only, not for diagnostic or therapeutic use.

Documentation
SPA-800PE Cert. of Analysis Lot 12040714
Spec Sheet

Product Manual

Product Name Catalog # Manual
Hsp27 Monoclonal Antibody (G3.1), R-Phycoerythrin Conjugate SPA-800PEE   MSDS   

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Citations
Launch Citation Database

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