TNF-alpha (rat) EIA Kit

Brand: Assay Designs

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Description	Size	Catalog#	Price	Quantity
TNF-alpha (rat) EIA Kit	1x96 well	900-086A	$495.00

Background

Tumor necrosis factor alpha (TNFa), also known as cachectin, is a 17.5 kDa 157 amino acid member of the TNF superfamily of cytokines that is a potent lymphoid factor with effects on a wide range of target cells. Active TNFa is produced in both soluble and membrane-anchored trimers by macrophages, NK cells, and T- and B-lymphocytes. TNF exerts proinflammatory signals via binding and inducing trimerization of TNF-receptor1 (TNF-R1) expressed on most normal and transformed cells, or to TNF-receptor 2 (TNF-R2), expressed on endothelial and most immune cells. TNF signaling regulates hematopeiesis, differentation, endothelial cell activation, apoptosis, lipid metabolism, tumor progression, and immune suveillance, and dysregulation of TNF or its receptors is implicated in numerous disease states including cancer, osteoporosis, autoimmune disease, diabetes, and atherosclerosis.

Product Comments
Assay Designs rat TNF-alpha enzyme immunometric assay kit is a complete kit for the quantitative determination of rat TNF-alpha in culture supernates with up to 10% serum supplement.

Applications
EIA

Format
ELISA

Features

Sensitive ELISA for the quantification of TNF alpha in rat culture supernates
Fast results in just 3 hours
Validated in culture supernates +/- 10% FBS

Range
31.3 - 2,000 pg/mL

Sample Size
100 無

Cross Reactivities
rat TNF-alpha 100%
No significant reactivity was observed with human, mouse, or porcine TNF alpha or human TNF beta

Sample Types
culture supernates

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
12.0 pg/mL in Assay Buffer; 26.7 pg/mL in culture media supplemented with 10% FBS

Species Reactivity
R

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout
Kit Insert
MSDS
Technical Bulletin

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TMB Substrate	80-0350
TNF-R1 Polyclonal Antibody	CSA-815E
TNF-alpha (human) EIA Kit	900-099
TNF-alpha (mouse) EIA Kit	900-047
Wash Buffer Concentrate	80-1287

Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134

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TNF-alpha (rat) EIA Kit