Hsp47 (Colligin) Recombinant Protein

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
Hsp47 (Colligin) Recombinant Protein  50 µg SPP-535D $135.00  
Hsp47 (Colligin) Recombinant Protein  200 µg SPP-535F $330.00  
Background
Hsp47 represents a novel 47-kDa stress glycoprotein expressed in a highly tissue- and cell-specific manner in primarily phenotypically altered collagen-producing cells. Prior to secretion, procollagen molecules achieve correct folding into triple helices in the ER, where Hsp47 specifically associates with procollagen during its folding and/or modification processes and functions as a collagen-specific molecular chaperone. Although the structural requirements for structural recognition and regulation of the binding remain unclear, Hsp47 may dissociate from procollagen during the process of prolyl 4-hydroxylation in the ER3. The expression of Hsp47 always occurs during the process of fibrosis in both humans and experimental animals particularly around the fibrotic lesions, suggesting that upregulation of Hsp47 may play an important role in the subsequent fibrotic process by regulating increased synthesis and assembly of collagens. Analysis of the amino acid sequence confirms Hsp47's unique place in the serpin superfamily, retained in the ER by its COOH-terminus RDEL peptide sequence while members like alpha 1-antitrypsin, antithrombin and ovalbumin are exported. Hsp47's residence in the ER does not prevent this chaperone's exportation into pre-Golgi vesicles, however. Hsp47 remains associated with procollagen up to the cis-Golgi compartment, where it dissociates from its substrate in a pH-independent but concentration-dependent manner, and is then recycled back to the ER. Despite the absence of an identified specific receptor for the RDEL COOH-terminal retention sequence of Hsp47, data indicates that Hsp47 is distributed, coprecipitates with and binds to the KDEL receptor (erd2 gene product). Under conditions of stress and alterations of intra-cellular pH (pHi), it also appears that significant levels of hsp47 elude erd2P retention and escape to the cell surface, potentially functioning as a serpin protein by modulating cell migration during the development, invasion and metastasis of cancer.

Species Reactivity
not applicable

Applications
WB Control

Format
Tris, pH 8.0, 100mM NaCl, 1mM DTT, 0.4M arginine

Purification Method
Denaturing conditions from the inclusion body fraction

Host Species
Recombinant human Hsp47 expressed in E. coli

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

Product Manual

Product Name Catalog # Manual
Hsp47 (Colligin) Recombinant Protein SPP-535D  
Hsp47 (Colligin) Recombinant Protein SPP-535F  

Related Products

Product Name Catalog#
Hsp47 (Colligin) Monoclonal Antibody (M16.10A1)
 		SPA-470

Citations
Launch Citation Database

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