Hsp27 (phospho-Ser78) EIA Kit

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Description	Size	Catalog#	Price	Quantity
Hsp27 (phospho-Ser78) EIA Kit	1x96 well	900-165	$525.00

Background

Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Table of Abbreviations

Applications
EIA

Features

Ultra-sensitive specific ELISA for Hsp27 phosphorylated at Serine78
Fast results in just 3 hours!
Fully quantitative results surpass semi-quantitative Western blot analysis
Validated with stimulation & inhibition cell lysate experiment

Range
31.25 - 1000 pg/mL

Sample Size
100 無

Sensitivity
4.30 pg/mL

Sample Types
cell lysates, serum, plasma

Samples Per Kit
40 in duplicate per 96 well kit

Species Reactivity
H

Time To Answer
3 hours

Cross Reactivities
Phospho Hsp27 (Ser78) 100%... Hsp27 (non-phospho) 0.1 % / Hsp25, Hsp40, Hsp70, Hsp90, AlphaB Crystallin, AlphaA Crystallin, <0.03%

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout
Kit Insert
MSDS

Related Products

Product Name	Catalog#
5X Extraction Reagent, 10 mL	80-1526
Assay Buffer 27, 100 mL	80-1753
Hsp27 (phospho) Recombinant Protein	SPP-716
Hsp27 (phospho-Ser15) EIA Kit	900-170
Hsp27 (phospho-Ser15) Polyclonal Antibody	SPA-525
Hsp27 (phospho-Ser78) Polyclonal Antibody	SPA-523
Hsp27 (phospho-Ser82) Polyclonal Antibody	SPA-524
Hsp27 ELISA Kit	EKS-500
Hsp27 High Sensitivity ImmunoSet	960-076
Hsp27 Monoclonal Antibody (G3.1)	SPA-800
Hsp27 Monoclonal Antibody (G3.1), FITC Conjugate	SPA-800FI
Hsp27 Recombinant Protein	SPP-715
Stop Solution 2, 10 mL	80-0377
TMB Substrate, 10 mL	80-0350
Wash Buffer Concentrate, 100 mL	80-1287

Citations
Launch Citation Database

Ordering Info
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Hsp27 (phospho-Ser78) EIA Kit