Hsp90 Monoclonal Antibody (16F1), Biotin Conjugate

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
Hsp90 Monoclonal Antibody (16F1), Biotin Conjugate  100 µg SPA-835BE $295.00  
Background
The Hsp90 family of proteins are highly conserved and found in all species. In unstressed cells, Hsp90 can account for 1-2% of the total cellular protein and this level increases when cells are exposed to heat shock or other stressors. Under heat shock conditions, high levels of Hsp90 are required for efficient reactivation of heat-denatured proteins, whereas under normal conditions Hsp90 is required by a specific group of substrate proteins. Mammalian Hsp90 generally exists as dimers and shares many functional similarities with Hsp70. Other chaperones are frequently found in the Hsp90-complex in the cytoplasm and forms an integral part of a ubiquitous multi-component chaperone system. Hsp90 is involved in many signal transduction pathways, including glucocorticoid receptor, progesterone receptor, estrogen receptor, dioxin receptor, and protein kinases. Members of the Hsp90 family are highly conserved in the N-terminal and C terminal regions. Studies have shown that these terminal regions have independent chaperone functions with different substrate specificities. The C-terminal has the ATP-independent chaperone activity for substrates, binding to partially folded polypeptides in an ATP-independent manner. The N-terminal domain binds to peptides longer than 10 amino acids, ATP, and to the selective Hsp90 inhibitor, Geldanamycin.

Product Comments
Assay Designs'TM Hsp90 Monoclonal Antibody (16F1), Biotin Conjugate detects an ~90 kDa protein in SDS-PAGE immunoblots in samples from human, mouse, and rat origins. It detects both the alpha and beta isoforms of Hsp90. This antibody only recognizes high molecular weight degradation fragments, suggesting an epitope which may be complex and easily destroyed by proteolysis.

Applications
WB

Format
PBS, 50% glycerol, 0.09% azide

Clone Number
16F1

Host Species
Rat

Immunogen
Native human Hsp90 protein

Species Reactivity
H, M, R

For research use only, not for diagnostic or therapeutic use.

Documentation
SPA-835B Cert. of Analysis Lot 08010709
Spec Sheet

Product Manual

Product Name Catalog # Manual
Hsp90 Monoclonal Antibody (16F1), Biotin Conjugate SPA-835BE  

Related Products

Product Name Catalog#
Geldanamycin
 		HPK-102J
Hsp90 Monoclonal Antibody (16F1)
 		SPA-835

Citations
Launch Citation Database

Ordering Info
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