Nitric Oxide Synthase Detection Reagent

Product Comments
THIS PRODUCT IS NOT FOR SALE IN JAPAN

Assay Designs' Nitric Oxide Synthase Detection Reagent provides a simple method for the real time detection of free nitric oxide and NOS activity in living cells under physiological conditions and may also be used with fresh or frozen tissue sections. This versatile detection reagent can be adapted for use in high throughput fluorescent plate-based assays, flow cytometry, or immunofluorescent microscopy. This reagent is not species specific and can also be used to detect NOS activity in plant cells.

Applications
Flow, IF

Product Image(s): click to enlarge

Format
Fluorescence

Features

• Detect NOS activity in real-time
• Easy-to-use cell permeable dye
• Quick No-Wash Assay
• Adaptable to High Throughput Screening
• Flexible use in multiple fluorescent applications

Sample Types
cells

Significance and Uses
Nitric oxide (NO), a diffusible free radical gas, affects a variety of physiological functions. NO acts as a neurotransmitter in the brain and peripheral nervous systems. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilation by releasing NO from the endothelium. NO has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neuronal regulation of smooth muscle including peristalsis, and penile erections. In macrophages, NO mediates tumoricidal and bactericidal actions. It mediates the actions of the excitatory neurotransmitter glutamate in stimulation of cGMP levels. Unlike typical neurotransmitters, NO is not stored in synaptic vesicles and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. NO is synthesized from L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS I-III, molecular weight ~130-160 kDa). One group of enzymes is constitutive, agonist-triggered, dependent on Ca2+/calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain, and platelets. The other principle group is inducible, Ca2+/calmodulin-independent, and also inhibited by NMMA and L-NNA. It has been found in macrophages, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analysis of cDNA clones has identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II).

Species Reactivity
Species Independent

For research use only, not for diagnostic or therapeutic use.