Hsp27 ELISA Kit

Brand: Stressgen

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Description	Size	Catalog#	Price	Quantity
Hsp27 ELISA Kit	1x96 well	EKS-500	$575.00

Background

Human Hsp27, mouse Hsp25 and alpha/beta-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. The state of phosphorylation and oligomerization of Hsp27 has been suggested to regulate microfilament organization because data demonstrates that only the non-phosphorylated lower molecular weight forms of Hsp27 bind actin barbed ends and inhibit polymerization. Hsp27 is also believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate a novel function for Hsp27 which is that Hsp27 is a negative regulator of cytochrome c-dependent activation of procaspase-3.

Table of Abbreviations

Applications
EIA

Format
ELISA

Range
0.39 - 25 ng/mL

Sample Size
100無/well

Product Image(s): click to enlarge

Sample Types
cell lysates, tissue extracts, serum, plasma

Samples Per Kit
39 samples in duplicate per 96 well kit

Sensitivity
0.39 ng/mL

Species Reactivity
H

Time To Answer
< 3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert

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Sample Diluent	80-1783
Stop Solution 2	80-0377
TMB Substrate	80-0350
Wash Buffer Concentrate	80-1287

Citations
Launch Citation Database

Ordering Info
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Hsp27 ELISA Kit