cyclic AMP EIA Kit

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Description Size Catalog# Price Quantity  
cyclic AMP EIA Kit  1x96 well 900-067 $320.00  
cyclic AMP EIA Kit  5x96 well 901-067 $1,275.00  
Background
Adenosine 3', 5'-cyclic monophosphate (cyclic AMP; cAMP) is one of the most important "second messengers" involved as a modulator of physiological processes. cyclic AMP is also involved in regulating neuronal, glandular, cardiovascular, immune and other functions and actions. A number of hormones are known to activate cyclic AMP through the action of the enzyme adenylate cyclase which converts ATP to cyclic AMP. These hormones include a variety of anterior pituitary peptide hormones such as corticotropin (ACTH), glucagon, calcitonin, thyroid stimulating hormone (TSH) and luteinizing hormone (LH). cyclic AMP has been shown to be involved in the cardiovascular, nervous system, and immune mechanisms, cell growth and differentiation, and general metabolism. There remains considerable interest in the measurement of intracellular cyclic AMP in tissues and cell cultures, and this may help to provide an understanding of the physiology and pathology of many disease states.

Product Comments
The cAMP kit is a competitive immunoassay for the quantitative determination of cyclic AMP in biological fluids. Sample types include culture supernates, saliva, and serum.

Applications
EIA

Format
ELISA

Features

  • Highly cited in peer reviewed literature
  • Can be run acetylated to increase sensitivity
  • Results in just 3 hours

Range
(non-acetylated) 0.78 - 200pmol/mL; (acetylated) 0.078 - 20pmol/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
cyclic AMP 100%
AMP 0.33%
ATP 0.12%
cyclic GMP <0.001%
GMP <0.001%
GTP <0.001%
cyclic UMP <0.001%
CTP <0.001%

Sample Types
culture media, serum, saliva, urine

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
(non-acetylated) 0.30 pmol/mL; (acetylated) 0.039 pmol/mL

Significance and Uses
Assay Designs' Regulart cyclic AMP (cAMP) kit is designed for the quantitative determination of cAMP in culture supernates, saliva, and serum. An optional acetylated protocol is available to increase sensitivity when needed.

Species Reactivity
Species Independent

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name Catalog # Manual
cyclic AMP EIA Kit 900-067   Kit Insert    MSDS   
cyclic AMP EIA Kit 901-067   Kit Insert    MSDS   

Ordering Info
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Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134