cyclic AMP Complete EIA Kit

Brand: Assay Designs

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Description	Size	Catalog#	Price	Quantity
cyclic AMP Complete EIA Kit	1x96 well	900-163	$350.00
cyclic AMP Complete EIA Kit	5x96 well	901-163	$1,450.00

Background

Adenosine 3', 5'-cyclic monophosphate (cyclic AMP; cAMP) is one of the most important "second messengers" involved as a modulator of physiological processes. cyclic AMP is also involved in regulating neuronal, glandular, cardiovascular, immune and other functions and actions. A number of hormones are known to activate cyclic AMP through the action of the enzyme adenylate cyclase which converts ATP to cyclic AMP. These hormones include a variety of anterior pituitary peptide hormones such as corticotropin (ACTH), glucagon, calcitonin, thyroid stimulating hormone (TSH) and luteinizing hormone (LH). cyclic AMP has been shown to be involved in the cardiovascular, nervous system, and immune mechanisms, cell growth and differentiation, and general metabolism. There remains considerable interest in the measurement of intracellular cyclic AMP in tissues and cell cultures, and this may help to provide an understanding of the physiology and pathology of many disease states.

Applications
EIA

Format
ELISA

Features

Accurately quantitate cAMP in biological fluids, cells, and tissue with one assay.

Acetylation option increases sensitivity 10-fold.

Ready-to-use liquid color coded reagents reduce technician error while saving time and money.

Range
0.78 - 200 pmol/mL (non-acetylated); 0.078 - 20 pmol/mL (acetylated)

Cross Reactivities
cAMP 100%, AMP <0.33%, ATP <0.12%, cGMP, GMP, GTP, cUMP, CTP <0.001%

Sample Types
tissue culture media, saliva, serum

Samples Per Kit
39 samples in duplicate per 96 wells

Sensitivity
Assay Buffer : 0.30 pmol/mL (non-acetylated) 0.039 pmol/mL (acetylated) HCl: 0.39 pmol/mL (non-acetylated) 0.037 pmol/mL (acetylated)

Significance and Uses
Assay Designs' Direct cyclic AMP (cAMP) kit is designed for use with cell culture and tissue. The kit includes 0.1M HCl which is used to lyse the cells, stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated

Species Reactivity
Species Independent

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout

Product Manual

Product Name	Catalog #	Manual
cyclic AMP Complete EIA Kit	900-163	Kit Insert MSDS
cyclic AMP Complete EIA Kit	901-163	Kit Insert MSDS

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Product Name	Catalog#
Assay Buffer 2 Concentrate, 100 mL	80-0074
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ERK1/2 (phospho) EIA Kit	900-098
ERK1/2 (total) EIA Kit	900-152
PGE1 EIA Kit	900-005
PGE1 EIA Kit	901-005
Stop Solution 2	80-0377
TMB Substrate	80-0350
Wash Buffer Concentrate	80-1287
cyclic AMP (direct) EIA Kit	900-066
cyclic AMP (direct) EIA Kit	900-066
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cyclic AMP EIA Kit	900-067
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Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134

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cyclic AMP Complete EIA Kit