Serotonin Receptor 5HT1A Polyclonal Antibody

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Description Size Catalog# Price Quantity  
Serotonin Receptor 5HT1A Polyclonal Antibody 100 µL  905-741-100 $295.00
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Assay Designs™ is the exclusive worldwide distributor of Proteimax activation-state specific GPCR antibodies.

Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Seven subclasses (5-HT 1-7) of serotonin receptors have been identified, a majority of which are members of the seven transmembrane domain-containing G-protein coupled receptor (GPCR) family, and are the target of several anti-depressant drugs. The 5-HT1 subclass consists of five highly homologous receptors that couple via G(i/o) alpha subunits to inhibit adenylyl cyclase activity. The 5-HT1A receptor gene is 99% homologous between human and mouse, and is expressed from an intronless gene with a predicted length of 421 amino acids. 5-HT1A receptor activation is generally inhibitory to cAMP production, but may also stimulate type II adenylyl cyclase activity in a cell-specific manner via release of G(beta/gamma) subunits when coupled to G(i2). 5-HT1A expression in mammals is strongest in CNS limbic pathways that control emotion, including tissue of the hippocampus, dorsal raphé, and septum.

Product Comments
Serotonin 5HT1A Receptor Activation-state Specific Polyclonal Antibody detects the active form of the receptor in IHC and ELISA, and detects the ~46 kDa protein in Western blots. The protein migration in Western blots can vary as a function of post-translation modifications. Protocols for the use of this product can be found in the technical resource section of our website.

Applications
IHC, WB, Membrane ELISA

Product Image(s): click to enlarge

Species Reactivity
M, R

Format
PBS, 50% glycerol, 0.01% sodium azide

Host Species
rabbit

Immunogen
Synthetic peptide derived from sequence on the amino-terminus of mouse Serotonin Receptor 5-HT1A

Purification Method
Peptide Affinity

Significance and Uses
Conformation-specific antibodies to GPCRs have been shown to distinguish between ligand bound and unbound receptors by preferentially binding to conformation state sensitive N-terminal epitopes exposed in the presence of receptor agonists.

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

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Citations
Launch Citation Database

Ordering Info
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