ERK1/2 (total) EIA Kit

Applications
EIA

Format
ELISA

Features

• Results in just 3 hours
• Sensitive to 194 pg/mL

Range
156 - 20,000 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge


Cross Reactivities
ERK1, active 100%
ERK2, active 100%
ERK1, inactive 100%
ERK2, inactive 92.6%
<0.08%: MEK1, inactive / MEK2, inactive / p38 alpha, inactive / JNK alpha1alpha, inactive

Sample Types
cell lysates

Samples Per Kit
38 in duplicate per 96 well kit

Sensitivity
22.9 pg/mL

Significance and Uses
The mitogen-activated protein kinase (MAPK) pathway exists in all eukaryotes. It consists of several subgroups including ERK 1/2 (extracellular signal-regulated kinase), JNK and p38 kinases. These kinases regulate and control fundamental cellular processes including proliferation, apoptosis, survival and differentiation. They are activated by diverse stimuli including cytokines, growth factors, irradiation, changes in osmolarity, heat and sheer stress. MAP kinases are characterized by their requirement for dual phosphorylation for full activation. ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif . The literature contains numerous and extensive reviews on MAP kinase.

Species Reactivity
H, M, R

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.