Big Endothelin-1 (human) EIA Kit

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Description Size Catalog# Price Quantity  
Big Endothelin-1 (human) EIA Kit 1x96 well  900-022 $460.00
Background
Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.

Table of Abbreviations

Applications
EIA

Format
ELISA

Features

  • Non-radioactive determination of Big ET-1
  • Easier than Western blotting techniques

Range
0.78 - 100 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
Big Endothelin-1 100%
rat Big Endothelin-1 100%
Endothelin-1(1-31) 57.6%
<0.10%: human Big Endothelin-2, human Big Endothelin-3, Endothelin-1 (1-21), Endothelin-2 (1-21), Endothelin-3 (1-21), Endothelin-2 (1-31), Endothelin-3 (1-31), VIC

Sample Types
culture media, serum

Samples Per Kit
38 in duplicate per 96 well kit

Sensitivity
0.23 pg/mL

Species Reactivity
H

Time To Answer
overnight

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout
Kit Insert
MSDS

Related Products

Product Name Catalog#
Big Endothelin-1 (rat) EIA Kit
 		900-073
Endothelin-1 EIA Kit
 		900-020A
Endothelin-A (ET-A) Receptor Polyclonal Antibody
 		915-006
Endothelin-A (ET-A) Receptor Polyclonal Antibody
 		915-007
Endothelin-B (ET-B) Receptor (B27) Polyclonal Antibody
 		915-008
Hydrogen Peroxide Chemiluminescent Detection Kit
 		907-012
Hydrogen Peroxide Colorimetric Detection Kit
 		907-015

Citations
Launch Citation Database

Ordering Info
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