Leptin (rat) EIA Kit

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Description Size Catalog# Price Quantity  
Leptin (rat) EIA Kit 1x96 well  900-015A $425.00
Background
The adipokine leptin is a hormone secreted predominantly by adipose tissue that signals through leptin receptors in the hypothalamus to decrease appetite and increase energy expenditure. Binding of leptin to the long-form of the leptin receptor in the hypothalamus reduces neuropeptide Y (NPY) and agouti-related protein (AgRP) activity, while stimulating anorexigenic pro-opiomelanocortin (POMC) neuron activity to reduce appetite. In peripheral tissues, leptin antagonizes insulin signaling, increases fatty acid oxidation, decreases insulin production in pancreatic beta cells, and promotes fertility. Its expression in adipocytes can be regulated in a paracrine fashion by other adipokines such as IL-6 (stimulation) and TNF-alpha (inhibition). Leptin deficient mice display severe insulin resistance, obesity, and decreased fertility, all of which are reversible by administration of exogenous leptin. In contrast, elevated circulating levels of leptin are associated with increased obesity, indicative of an acclimated state of leptin resistance which is not well understood.

Table of Abbreviations

Applications
EIA

Features

  • No edge effect - all incubations at room temperature eliminate risk of evaporation at 37ēC
  • Broad dynamic range - easily quantify high and low levels of leptin
  • Convenient- store all reagents at 4ēC

Range
100 - 6,400 pg/mL

Product Image(s): click to enlarge

Cross Reactivities
Rat Leptin 100% / Mouse Leptin 318% / Human Leptin 0.5%

Sample Types
CS, P, S

Samples Per Kit
39 in dupliate

Sensitivity
67.2 pg/mL

Species Reactivity
R

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert
MSDS
Technical Bulletin

Related Products

Product Name Catalog#
Leptin (human) EIA Kit
 		900-028A
Leptin (mouse) EIA Kit
 		900-019A

Citations
Launch Citation Database

Ordering Info
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