Akt1,2 Polyclonal Antibody

Brand: Assay Designs Print Page
Description Size Catalog# Price Quantity  
Akt1,2 Polyclonal Antibody 100 µg  905-755-100 $295.00
Background
The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK3a, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has recently been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.

Product Comments
Akt Polyclonal Antibody detects an ~56kDa protein in SDS-PAGE immunoblots corresponding to the apparent molecular mass of Akt1, 2 in samples from human, mouse, and rat origins.

Applications
WB

Product Image(s): click to enlarge

Species Reactivity
H, M, R

Format
PBS, pH 7.2, 0.09% azide, 50% glycerol

Host Species
Rabbit

Immunogen
Synthetic peptide derived from the C-terminus of human Akt

Purification Method
Protein A Affinity

Positive Control
A431, PC-12, and NIH-3T3 cell lysates

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

Related Products

Product Name Catalog#
Akt (PKB) Kinase Activity Kit
 		EKS-400A
Akt (phospho-Ser473) Polyclonal Antibody
 		905-773-100
Akt (phospho-Thr308) Polyclonal Antibody
 		KAP-PK007E
Akt Polyclonal Antibody
 		KAP-PK004
Akt1 (PKB alpha) Monoclonal Antibody (5G12)
 		905-668
Akt1,2 (phospho-Ser473) EIA Kit
 		900-162
Akt1,2,3 (phospho-Ser473) Monoclonal Antibody (11E6)
 		905-658
Akt1,2,3 Monoclonal Antibody (11A11)
 		905-659
Akt1,2,3 Monoclonal Antibody (5C10)
 		905-661
Akt2 (PKB beta) Monoclonal Antibody (8B7)
 		905-660
Akt2 Polyclonal Antibody
 		KAP-PK008E
Hsp90 alpha ELISA Kit
 		EKS-895
PI3-kinase, p85 alpha Monoclonal Antibody (AB6)
 		KAM-PI200E

Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
 Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134