Akt1,2,3 (phospho-Ser473) Monoclonal Antibody (11E6)

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Description Size Catalog# Price Quantity  
Akt1,2,3 (phospho-Ser473) Monoclonal Antibody (11E6) 100 µg  905-658 $395.00
Background
The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3beta, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has recently been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.

Table of Abbreviations

Applications
EIA, Flow, IHC, WB

Format
Purified

Product Image(s): click to enlarge

Clone Number
11E6

Host Species
mouse

Immunogen
Synthetic phospho-peptide derived from the sequence of Akt 1,2,3, conjugated to KLH

Species Reactivity
H, M, R, C

Positive Control
Pervanadate-treated HepG2 cells

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

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Citations
Launch Citation Database

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