Endothelin-1 EIA Kit

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Description Size Catalog# Price Quantity  
Endothelin-1 EIA Kit 1x96 well  900-020A $450.00
Background
The discovery by Rubanyi's group in 1985 of a peptide termed endothelium-derived contracting factor (EDCF) and the isolation, sequencing, cloning and naming endothelin by Yanagisawa of the most potent vasoconstrictor has led to greater understanding of many physiological effects. Endothelin-1 (ET-1) is a 21-amino acid peptide, that with 2 other isopeptides, have been shown to be derived from the expression of three separate genes. The genes make three pre-propeptides that are cleaved into three different big endothelin molecules, each 39 amino acids long. These propeptides are cleaved by endothelin-converting enzyme into the three peptides, ET-1, ET-2 and ET-3. A number of reviews of the structure, function, and molecular biology of the endothelin family of peptides and propeptides are available. ET-1 has been shown to have potent effects on smooth muscle cells, fibroblasts and to be involved in many disease processes, particularly cardiovascular diseases. It has been shown to be important in congestive heart failure, renal failure, and pulmonary hypertension. In a 1995 report it has also been shown to be elevated in the plasma of patients with metastatic prostate cancer.

Applications
EIA

Format
ELISA

Product Image(s): click to enlarge

Features

  • Non-radioactive determination of ET-1
  • Results in just 2 hours
  • Convenient 4°C storage

Range
0.78 - 100 pg/mL

Sample Size
100 µL

Cross Reactivities
ET-1 100%; ET-2 21%; ET-3 3.6 %, Big ET-1, Big ET-2, Big ET-3 < 0.1%

Sample Types
culture supernates, serum, plasma

Samples Per Kit
38 in duplicate per 96 well kit

Sensitivity
0.40 pg/mL

Species Reactivity
H, M, R, B, C, P, RB

Time To Answer
2 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout
Kit Insert
MSDS
Technical Bulletin

Related Products

Product Name Catalog#
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Big Endothelin-1 (human) EIA Kit
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Big Endothelin-1 (rat) EIA Kit
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Endothelin-A (ET-A) Receptor Polyclonal Antibody
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Endothelin-A (ET-A) Receptor Polyclonal Antibody
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Endothelin-B (ET-B) Receptor (B27) Polyclonal Antibody
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Hydrogen Peroxide Chemiluminescent Detection Kit
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Stop Solution 2
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Citations
Launch Citation Database

Ordering Info
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