PKC Substrate

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
PKC Substrate 1mg  SPK-104J $150.00
Background
Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties: classical or conventional PKC isotypes (cPKC), including PKC-alpha, -beta-I, -beta-II, and -gamma; novel PKC isotypes (nPKC), including PKC-delta, -epsilon, -eta, and -theta; and atypical PKC isotypes (aPKC), including PKC-zeta and -lambda/iota. All PKC isotypes share a characteristic amino terminal C1 regulatory domain linked to a catalytic serine/threonine-protein kinase domain. The cPKC isotypes contain two conserved C1 and C2 domains in the cPKC regulatory domain. The cPKC-C1 domains contain a repeat of a cysteine-rich sequence, C1A and C1B, each of which tightly bind two zinc ions and constitute a binding site for diacylglycerols (DAGs) and phorbol esters. The cPKC-C2 domains are Ca2+-dependent phospholipid binding domains that show specificity to acidic phospholipids such as phosphatidylserine. nPKC isotypes, like cPKCs, bind DAG and phorbol ester but lack a functional C2 domain, and thus do not require Ca2+ for activation. The aPKC isotypes lack the entire C2 domain and one cysteine-rich loop in the C1 domain, making their activation independent of DAG and Ca2+.

Product Comments
This peptide is an appropriate substrate for Assay Designs' Active PKCm (product# PPK-450) or Active PKCn (product# PPK-457)

Species Reactivity
Species Independent

Applications
KA

Format
Lyophilized

Purity
>95% (determined by HPLC)

Source
Synthetic PKC substrate peptide (QKRPSQRSKYL)

For research use only, not for diagnostic or therapeutic use.

Documentation
TechSpec

Related Products

Product Name Catalog#
MARCKS (phospho-Ser152/156) Polyclonal Antibody
 		KAP-ST016E
PKC mu Active Recombinant Protein
 		PPK-450
PKC nu Active Recombinant Protein
 		PPK-457

Citations
Launch Citation Database

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