Nitric Oxide Synthase Detection Reagent

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Description Size Catalog# Price Quantity  
Nitric Oxide Synthase Detection Reagent 0.125 mg  906-042 $135.00
Background
The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.

Table of Abbreviations

Applications
Flow, IF

Product Image(s): click to enlarge

Format
Fluorescence

Features

  • Detect NOS activity in real-time
  • Easy-to-use cell permeable dye
  • Quick No-Wash Assay
  • Adaptable to High Throughput Screening
  • Flexible use in multiple fluorescent applications

Sample Types
cells

Species Reactivity
Species Independent

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert
MSDS

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Citations
Launch Citation Database

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