Bim/BOD Polyclonal Antibody

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
Bim/BOD Polyclonal Antibody  100 µg AAP-330E $285.00  
Background
The Bcl-2 interacting mediator of cell death (Bim/BOD) is a critical regulator of lymphocyte homeostasis, a barrier against autoimmune disease, and an essential player in the apoptotic response to certain stress signals. BimEL, BimL and BimS, three Bim splice variants with apparent molecular masses of ~23, 16, and 13 kDa, respectively, have been identified. All isoforms of Bim contain the Cterminal Bcl-2 homology domain (BH3) found in all apoptotic proteins in the Bcl-2 family, including Bad, Bax, Bid, Bik, Hrk and Bim/BOD. Of the three isoforms, BimS is the most potent inducer of apoptosis. The three isoforms bind an 8 kDa dynein light chain, LC8 (also known as PIN or Dlc-1), which mediates the function of BimEL and BimL. BimEL and BimL are sequestered to this microtubular dynein motor complex by binding LC8 in the cytosol. Certain apoptotic stimuli release Bim from the complex, allowing it to translocate to the mitochondria and bind to Bcl-2 or its homologues, thereby promoting apoptosis. Bim is downregulated by either of two Ras pathways (Raf/MAPK or PI3-K/mTOR). Bim downregulation is a critical step in the maintenance of hematopoietic cell survival, while activation of Bim is a primary trigger for negative selection of autoreactive T cells during their development in the thymus.

Product Comments
This antibody recognizes an epitope that is common to all three Bim isoforms which are BimEL (~23 kDa), BimL (~16 kDa) and BimS (~13 kDa).

Applications
IHC, WB

Format
PBS, 0.02% sodium azide

Product Image(s): click to enlarge

Host Species
rabbit

Immunogen
Synthetic peptide derived from the sequence of human Bim/BOD, conjugated to KLH

Species Reactivity
H, M, R

Purification Method
Peptide Affinity

For research use only, not for diagnostic or therapeutic use.

Documentation
AAP-330 Cert. of Analysis Lot 02080821
AAP-330 Cert. of Analysis Lot 05240608
AAP-330 Cert. of Analysis Lot 09200603
AAP-330 Cert. of Analysis Lot 11190704
TechSpec

Product Manual

Product Name Catalog # Manual
Bim/BOD Polyclonal Antibody AAP-330E  

Related Products

Product Name Catalog#
Bax (human) EIA Kit
 		900-138
Bax Polyclonal Antibody
 		AAS-040
Bcl-2 (human total) EIA Kit
 		900-133
Bcl-2 Polyclonal Antibody
 		AAS-070
Bnip3L (IN) Polyclonal Antibody
 		905-185
mTOR Polyclonal Antibody
 		905-687-100

Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
 Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134