c-FLIP Polyclonal Antibody

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
c-FLIP Polyclonal Antibody  100 µg AAP-440E $255.00  
c-FLIP Polyclonal Antibody  25 µg AAP-440C $115.00  
Background
cFLIP (cellular FLICE-like inhibitory protein) is a cellular homologue of the viral protein, vFLIP, also known as CASH, Casper, CLARP, FLAME, I-FLICE, MRIT and Usurpin. cFLIP contains two death effector domains (DEDs) and is expressed as either a short or long splice variant called cFLIPs and cFLIPL, respectively. cFLIP can block Fas-mediated apoptosis presumably through the C-terminal region of cFLIPL which shares homology with the proteolytic domain of caspase-8 (FLICE). However, cFLIP lacks the active proteinase site required for catalytic activity. Therefore, although it can be recruited to the ligated Fas-signaling complex, it cannot deliver a death signal. cFLIP is expressed in the cytosol, and cells with high levels of cFLIP relative to FLICE appear generally resistant to apoptosis, whereas cells with low levels of cFLIP relative to FLICE are more sensitive to apoptosis4. cFLIP acts as a caspase-8 dominant negative regulator, blocking apoptosis induced by the oligomerization of the adapter protein, FADD. cFLIP interacts with FADD and caspase-8 through their respective DEDs. The regulation and role of cFLIP in mediating signals that promote survival, such as NF-B, versus inhibiting cell death by blocking cell death receptor-induced caspase activation, is unclear.

Applications
ICC, WB

Format
PBS, 0.02% sodium azide

Host Species
Rabbit

Immunogen
Synthetic peptide derived from the sequence of human c-FLIP, conjugated to KLH; sequence conserved among all c-FLIP variants

Species Reactivity
H, M, R

Purification Method
Peptide Affinity

For research use only, not for diagnostic or therapeutic use.

Documentation
TechSpec

Product Manual

Product Name Catalog # Manual
c-FLIP Polyclonal Antibody AAP-440E  
c-FLIP Polyclonal Antibody AAP-440C  

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Citations
Launch Citation Database

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