Adrenergic Receptor Beta2 Polyclonal Antibody

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Description Size Catalog# Price Quantity  
Adrenergic Receptor Beta2 Polyclonal Antibody 100 µL  905-742-100 $295.00
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Assay Designs™ is the exclusive worldwide distributor of Proteimax activation-state specific GPCR antibodies.

Adrenergic receptors mediate the hormone and neurotransmitter functions of adrenaline and noradrenaline. Adrenoreceptors function in peripheral tissues and the central nervous system to regulate numerous vascular, metabolic, and neurological functions, and are the target for many therapeutically relevant drugs including those for the treatment of hypertension, asthma, benign prostatic hypertrophy, and depression1. Adrenoreceptors are classified by three types and multiple subclasses (alpha1A,1B,1D; alpha2A,2B,2C; and beta1,2,3) based on pharmacological and molecular characteristics. The 450 amino acid alpha2A receptor is most highly expressed in human brain, spleen, and kidney, and is a species orthologue of the alpha2D receptor expressed similarly in rat, mouse, and cow. Activated alpha2A receptors signal via Gi/o alpha subunits to inhibit adenylyl cyclase activity, increase potassium transport, inhibit calcium conductance, and stimulate phospholipase A2. The shorter (418 amino acid) beta2 receptor is expressed predominantly in the brain, lung, and heart. Agonist affinity to beta2 adrenergic receptors is modulated by Zn(2+) concentration, and in contrast to alpha2A receptors, beta2 receptor signaling is primarily coupled to GS alpha subunits, stimulating production of cAMP and activation of protein kinase A to regulate opening of L-type Ca(2+) channels. Beta2 receptors may also trigger cGMP accumulation and protein kinase G activation via Gi/o alpha subunit coupling in some cell types. Conformation-specific antibodies to adrenergic receptors alpha2A and beta2, as well as other GPCRs have been shown to distinguish between ligand bound and unbound receptors by preferentially binding to conformation state sensitive N-terminal epitopes exposed in the presence of receptor agonists.

Product Comments
The Beta2 Adrenergic Receptor Activation-state Specific Polyclonal Antibody detects the active form of the receptor in IHC and ELISA, and detects the ~47 kDa protein in Western blots. The protein migration in Western blots can vary as a function of post-translation modifications. Protocols for the use of this product can be found in the technical resource section of our website.

Applications
IHC, WB, Membrane ELISA

Product Image(s): click to enlarge

Species Reactivity
H, M, R

Format
PBS and 50% glycerol

Host Species
rabbit

Immunogen
Synthetic peptide derived from sequence on the amino-terminus of mouse beta2 Adrenergic Receptor

Purification Method
Peptide Affinity

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

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Citations
Launch Citation Database

Ordering Info
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Order by fax: 734-669-0134