Akt1 Recombinant Human Protein

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
Akt1 Recombinant Human Protein  15 µg PPK-410Y $320.00  
Akt1 Recombinant Human Protein  1 µg PPK-410X $100.00  
Background
The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3beta, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has recently been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.

Table of Abbreviations

Abbreviated Name
Akt1 Recomb Human Protein

Expression System
Active recombinant full length human Akt1 with an N-terminal GST-tag, expressed by baculovirus in Sf9 insect cells.

Species Reactivity
not applicable

Applications
AA

Purification
>90% pure as determined by densitometry.

Tag
GST

Source
Human

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For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

Product Manual

Product Name Catalog # Manual
Akt1 Recombinant Human Protein PPK-410Y  
Akt1 Recombinant Human Protein PPK-410X  

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Citations
Launch Citation Database

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