Caspase-3 Colorimetric Detection Kit

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Description Size Catalog# Price Quantity  
Caspase-3 Colorimetric Detection Kit 1x96 well  907-013 $360.00
Background
Caspase-3 is also known as Apopain, CPP-32 and Yama. It is an intracellular cysteine protease that exists as a proenzyme, becoming activated during the sequence of events associated with apoptosis. Caspase-3 cleaves a variety of cellular molecules that contain the amino acid theme DEVD such as poly ADP-ribose polymerase (PARP), the 70 kD protein of the U1-ribonucleoprotein and a subunit of the DNA dependent protein kinase. Apoptosis was originally reported in 1972 and was described as a mechanism of controlled or programmed cell death. This process is very common in bone marrow and organs with high proliferative activity. It has also been implicated in the progression of a number of diseases, including AIDS, cancer and autoimmune pathologies and has been extensively studied by cellular biologists in fas-mediated cell death. Apoptosis is characterized by a variety of changes including loss of cellular membrane phospholipid symmetry, chromatin condensation, mitochondrial swelling and eventually leads to damage and fragmentation of DNA. This process results in cell death distinctly different from necrosis. As a result, apoptotic cells avoid the inflammatory response normally associated with necrosis.

Table of Abbreviations

Format
Detection Kit

Features

  • Results in 3 hours

Range
76 - 933 Units/mL

Sample Size
50 µL

Product Image(s): click to enlarge

Samples Per Kit
37 in duplicate per 96 well kit

Sensitivity
16.4 Units/mL

Significance and Uses
Caspase-3 activation plays a central role in apoptosis, also known as programmed cell death. Activated caspase-3 is responsible for the breakdown of several cellular components related to DNA-repair and regulation. The measurement of capsase-3 allows for the study of apoptotic mechanisms.

Species Reactivity
Species Independent

Applications
Detection

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout
Kit Insert
MSDS

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Citations
Launch Citation Database

Ordering Info
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