Bax (human) EIA Kit

Applications
EIA

Format
ELISA

Features

• Only complete Bax immunoassaykit on the market
• Thoroughly validated in mitochondrial and cytoplasmic fractions

Range
62.5 - 2,000 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
human Bax 100%
p21 <0.7%
<0.1%: Bcl-2 / Bcl-XL / CDK4 / p27 / PCNA

Sample Types
cell lysates, HeLa cells

Samples Per Kit
40 in duplicate per 96 well kit

Sensitivity
10.1 pg/mL

Significance and Uses
Bax (Bcl-2 associated x protein) is an apoptosis regulating protein of the Bcl-2 family. The ratio of Bax to Bcl-2 determines survival or death following an apoptotic stimulus. Bax is implicated in many cancers, uterine leiomyomas, and brain dysmorphology. Bax is a 21 kDa protein that accelerates apoptosis by binding to, and antagonizing the death repressor activity of Bcl-2 in vivo. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol, in this manner activating caspase-3 and perpetuating the apoptotic cascade. Protein levels of Bax and other Bcl-2 family members are altered in various human malignancies, with changes in expression levels implicated in diseases involving apoptosis such as cancer, autoimmune diseases and neurodegenerative disorders.

Species Reactivity
H

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.