Bcl-2 (human total) EIA Kit

Table of Abbreviations

Applications
EIA

Format
ELISA

Features

• Most sensitive Bcl-2 immunoassay on the market
Kli>Thoroughly validated in cell lysates

Range
18.8-1,200 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
human Bcl-2 100%
<0.1%: Bad / Bax / Bid / Bcl-XL / cIAP1 / total JNK / pTEN / Smac/DIABLO / beta-catenin

Sample Types
Cell Lysis Buffer 2,2 million Jurkat cells per mL

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
3.8 pg/mL

Significance and Uses
Bcl-2 (B-cell lymphoma 2) is an oncogenic protein that inhibits apoptosis. It is located within the mitochondrial membrane, endoplasmic reticulum and nuclear envelope. Bcl-2 is encoded by a 230 kb gene that results in a 24-26 kDa protein. Bakhshi first identified Bcl-2 as an apoptotic proto-oncogene while studying chromosomal translocations in human follicular lymphomas. At approximately the same time Tsujimoto and Croce made the same observation. The function of Bcl-2 was first described by Vaux when it was demonstrated that it promoted the survival of factor dependent cells. Bcl-2 overexpression has been implicated in a wide variety of malignancies including (but not limited to) breast, prostate, skin, colon and pancreatic cancers. Related anti-apoptotic proteins include Bcl-xL, Mcl-1 and Bcl-w. Pro-apoptotic proteins in the Bcl-2 family include Bax, BAD, Bak, Blk and Bid.

Species Reactivity
H

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.