8-iso-PGF2alpha (direct) EIA Kit

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Description Size Catalog# Price Quantity  
8-iso-PGF2alpha (direct) EIA Kit  1x96 well 900-091 $360.00  
8-iso-PGF2alpha (direct) EIA Kit  5x96 well 901-091 $1,395.00  
Background
The 8-epimer of Prostaglandin F2alpha (8-iso-PGF2alpha) is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependent mechanisms from arachidonic acid. 8-iso-PGF2alpha has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and atherosclerosis, and a mutagen in 3T3 and vascular smooth muscle cells. It has also been postulated to participate as a pathophysiological mediator, and is able to modify the fluidity and integrity of membranes. 8-iso-PGF2alpha has been shown to circulate in plasma and is excreted in urine. Methods for assessing total 8-iso-PGF2alpha typically require the alkaline hydrolysis of 8-iso-PGF2alpha esters from tissues, followed by lengthy procedures involving extractions, phase separations and thin layer chromatography.

Applications
EIA

Format
ELISA

Features

  • Non-radioactive measurement of 8-iso-PGF2a in serum and plasma
  • Two formats allow flexibility
  • Overnight format allows sensitivity down to 40 pg/mL

Range
160 - 100,000 pg/mL

Sample Size
50 µL

Product Image(s): click to enlarge

Cross Reactivities
8-iso-PGF2alpha 100%
PGF1alpha 4.6%
PGF2alpha 1.85%
PGE1 0.19%
TXB2 0.023%
PGB1 0.02%
PGE3 0.012%
6-keto-PGF1alpha 0.008%
13,14-dihydro-15-keto-PGF2alpha 0.008%
6,15-keto-13,14-dihydro-PGF1alpha 0.005%
8-iso-PGE1 <0.001%
PGA2 <0.001%
PGJ2 <0.001%
2-Arachidonoylglycerol <0.001%
Anandamide <0.001%

Sample Types
serum, plasma

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
(2 hour) 103.2 pg/mL; (overnight) 40.0 pg/mL

Significance and Uses
The direct 8-iso-Prostaglandin F2alpha (8-iso-PGF2alpha) kit is a competitive immunoassay for the quantitative determination of 8-iso-Prostaglandin F2alpha in biological fluids. Sample types include serum, heparin plasma, and EDTA plasma.

Species Reactivity
Species Independent

Time To Answer
(2 hour) <3 hours; (overnight) overnight

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name Catalog # Manual
8-iso-PGF2alpha (direct) EIA Kit 900-091   Kit Insert    MSDS   
8-iso-PGF2alpha (direct) EIA Kit 901-091   Kit Insert    MSDS   

Ordering Info
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