LTB4 EIA Kit

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Description Size Catalog# Price Quantity  
LTB4 EIA Kit   1x96 well 900-068 $345.00  
LTB4 EIA Kit   5x96 well 901-068 $1,395.00  
Background
The Assay Designs LTB4 ELISA is a robust, sensitive, and easy-to-use assay to quantitatively determine LTB4 levels in a variety of biological matrices.

Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.

Normalize urine samples to creatinine with our Creatinine Colorimetric Detection Kit.

Table of Abbreviations

Applications
EIA

Species Reactivity
Species Independent

Features

  • Versatile - validated in a wide variety of sample matrices
  • Fast - results in 4 hours
  • Accurate - reproducible results lot-after-lot

Range
11.7 - 3,000 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
LTB4 100%
6-trans-12-epi-LTB4 5.50%
6-trans-LTB4 4.90%
12-epi-LTB4 0.94%
<0.20%:20-OH-LTB4 / PGE2 / PGF2alpha / 20-COOH-LTB4 / LTC4 / LTD4 / LTE4 / 5(S)-HETE / 12(S)-HETE / 15(S)-HETE

Sample Types
culture media, saliva, urine, EDTA plasma

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
5.63 pg/mL

Time To Answer
4 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name Catalog # Manual
LTB4 EIA Kit 900-068   Kit Insert     MSDS    
LTB4 EIA Kit 901-068   Kit Insert     MSDS    

Related Products

Product Name Catalog#
12(S)-HETE EIA Kit
900-050
12(S)-HETE EIA Kit
901-050
13(S)-HODE EIA Kit
900-108
15(S)-HETE EIA Kit
900-051
15(S)-HETE EIA Kit
901-051
Creatinine Colorimetric Detection Kit
907-030
Cysteinyl Leukotriene EIA Kit
900-070
PGE2 EIA Kit
900-001
sPLA2 Activity Kit
907-002

Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
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Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134