15(S)-HETE EIA Kit

Brand: Assay Designs

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Description	Size	Catalog#	Price	Quantity
15(S)-HETE EIA Kit	1x96 well	900-051	$360.00
15(S)-HETE EIA Kit	5x96 well	901-051	$1,440.00

Background

HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.

Table of Abbreviations

Applications
EIA

Features

Most sensitive 15(S)-HETE kit on the market
Few preparation steps
Much easier than GC/MS measurement

Range
78.1 - 20,000 pg/mL

Sample Size
100 無

Product Image(s): click to enlarge

Cross Reactivities
15(S)-HETE 100%
8,15-diHETE 1.00%
5,15-diHETE 1.00%
13(S)-HODE 0.60%
5-HETE 0.10%
PGD2 0.10%
PGF2alpha 0.10%
PGB2 0.10%
12(S)-HETE <0.05%
Arachidonic Acid <0.05%
PGE2 <0.05%
12(R)-HETE <0.05%
Linoleic Acid <0.05%

Sample Types
culture media, urine, EDTA plasma, serum

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
69.21 pg/mL

Species Reactivity
Species Independent

Time To Answer
overnight

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name	Catalog #	Manual
15(S)-HETE EIA Kit	900-051	Kit Insert MSDS
15(S)-HETE EIA Kit	901-051	Kit Insert MSDS

Related Products

Product Name	Catalog#
12(S)-HETE EIA Kit	900-050
12(S)-HETE EIA Kit	901-050
13(S)-HODE EIA Kit	900-108
15(S)-HETE EIA Kit	900-051
15(S)-HETE EIA Kit	901-051
Creatinine Colorimetric Detection Kit	907-030
Cysteinyl Leukotriene EIA Kit	900-070
LTB4 EIA Kit	900-068
PGE2 EIA Kit	900-001
PGF2alpha EIA Kit	900-069
TXB2 EIA Kit	900-002

Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134

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15(S)-HETE EIA Kit