12(S)-HETE EIA Kit

Brand: Assay Designs

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Description	Size	Catalog#	Price	Quantity
12(S)-HETE EIA Kit	1x96 well	900-050	$360.00
12(S)-HETE EIA Kit	5x96 well	901-050	$1,440.00

Background

HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.

Table of Abbreviations

Applications
EIA

Format
ELISA

Features

Only 12(S)-HETE immunoassay on the market
Much easier than GC/MS measurement

Range
195 - 50,000 pg/mL

Sample Size
100 無

Product Image(s): click to enlarge

Cross Reactivities
12(S)-HETE 100%
12(R)-HETE 2.50%
15-HETE 0.30%
5(S)-HETE 0.20%
0.10%: 8,15-diHETE / 5,15-diHETE / PGE2 / PGF2alpha / PGD2 / 6-keto-PGF1alpha / TXB2 / Arachidonic Acid / LTB4 / LTC4 / LTD4 / LTE4
<0.1%: 8-HETE / 9-HETE / 11-HETE

Sample Types
culture media, plasma

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
146 pg/mL

Species Reactivity
Species Independent

Time To Answer
5 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name	Catalog #	Manual
12(S)-HETE EIA Kit	900-050	Kit Insert MSDS
12(S)-HETE EIA Kit	901-050	Kit Insert MSDS

Related Products

Product Name	Catalog#
12(L)-HETE Polyclonal Antibody	905-053
12-HETE Polyclonal Antibody	905-032
15(S)-HETE EIA Kit	900-051
Cysteinyl Leukotriene EIA Kit	900-070
LTB4 EIA Kit	900-068
PGE2 EIA Kit	900-001
PGF2alpha EIA Kit	900-069
TXB2 EIA Kit	900-002

Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134

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12(S)-HETE EIA Kit