Big Endothelin-1 (human) EIA Kit

Product Comments
The human Big Endothelin-1 kit is an immunometric assay for the quantitative determination of human big endothelin in biological fluids. Sample types include culture media, and serum.

Applications
EIA

Format
ELISA

Features

• Non-radioactive determination of Big ET-1
• Easier than Western blotting techniques

Range
0.78 - 100 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
Big Endothelin-1 100%
rat Big Endothelin-1 100%
Endothelin-1(1-31) 57.6%
<0.10%: human Big Endothelin-2, human Big Endothelin-3, Endothelin-1 (1-21), Endothelin-2 (1-21), Endothelin-3 (1-21), Endothelin-2 (1-31), Endothelin-3 (1-31), VIC

Sample Types
culture media, serum

Samples Per Kit
38 in duplicate per 96 well kit

Sensitivity
0.23 pg/mL

Significance and Uses
Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstriction activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. Vascular endothelial injury stimulates the release of endothelins, which activate macrophages and act as strong chemo-attractants for circulating monocytes. Endothelins affect vascular smooth muscle fibroblast proliferation; help regulate vascular, airway, and intestinal smooth muscle tone; increase the activity of bone alkaline phosphatase; stimulate release of atrial natriuretic peptide (ANP) from atrial cardiocytes; inhibit the release of renin from glomeruli and modulate norepinephrine at sympathetic nerve termini. Opposing vasomotor effects are regulated through the binding of distinct ET receptors for vasoconstriction and vasodilation. Endothelins have been linked to atherosclerosis and various cardiovascular disease states. ET-1 is important in congestive heart failure, renal failure, pulmonary hypertension, hyperlipidemia and metastatic prostate cancer. Big ET-1 is more stable than ET-1 in plasma and may be useful in assessing ECE inhibitors. It exerts similar effects in vivo, but has reduced vasoconstrictor activity in vitro.

Species Reactivity
H

Time To Answer
overnight

For research use only, not for diagnostic or therapeutic use.