Hsp70 High Sensitivity ELISA Kit

Brand: Stressgen

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Description	Size	Catalog#	Price	Quantity
Hsp70 High Sensitivity ELISA Kit	1x96 well	EKS-715	$575.00

Background

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Table of Abbreviations

Applications
EIA

Features

Ultra-sensitive ELISA for the measurement of Hsp70 in serum and EDTA plasma samples
Fast results in just 4.5 hours
Highly specific for inducible Hsp70 (Hsp72)

Range
0.20 - 12.5 ng/mL

Cross Reactivities
"Hsp72 (human) 100% ... Hsp72 (rat) 117.6% / Hsp70 (mouse from A2 gene, cytosolic) 33.6% / Hsp70 (salmon) 0.8% / Hsp70B' (human) 5.4% / DnaK (E.coli) 0.5% / Hsc70 (Bovine) <0.016% / Grp78 (hamster) <0.016% / Hsp71 (M. tuberculosis) <0.016%

Sample Types
serum, EDTA plasma

Samples Per Kit
39 in duplicate

Sensitivity
90 pg/mL

Species Reactivity
H, M, R

Time To Answer
4.5 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout
Kit Insert
MSDS

Related Products

Product Name	Catalog#
Hsp70 (Anti-Human IgG/A/M) ELISA	EKS-750
Hsp70 (Hsp72) Monoclonal Antibody (C92F3A-5), FITC Conjugate	SPA-810FI
Hsp70 (Hsp72) Polyclonal Antibody	SPA-811
Hsp70 ELISA Kit	EKS-700B
Hsp70B prime ELISA Kit	EKS-725
Stop Solution 2	80-0377
TMB Substrate	80-0350
Wash Buffer Concentrate	80-1287

Citations
Launch Citation Database

Ordering Info
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Hsp70 High Sensitivity ELISA Kit