Autophagy Protein 7 (APG7) Polyclonal Antibody

Product Comments
This peptide affinity purified Autophagy Protein 7 (APG7) Polyclonal Antibody is suitable for use in WB and ICC on samples from human and mouse origins. An ~76 kDa band is observed in SDS-PAGE immunoblots.

Applications
ICC, WB

Product Image(s): click to enlarge

Species Reactivity
H, M

Format
PBS, 0.02% sodium azide

Host Species
Rabbit

Immunogen
Synthetic peptide corresponding to a sequence near the carboxy-terminus of human APG7

Positive Control
Caco-2 and MCF7 Cell Lysates

Purification Method
Peptide Affinity

Significance and Uses
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components, and is negatively regulated by TOR (Target of Rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, and starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

For research use only, not for diagnostic or therapeutic use.