Crystallin, AlphaA Polyclonal Antibody

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
Crystallin, AlphaA Polyclonal Antibody  200 µL SPA-221F $325.00  
Crystallin, AlphaA Polyclonal Antibody  50 µL SPA-221D $140.00  
Background
Composed of ~20 kDa alphaA and alphaB subunits, alpha-crystallins function as major water-soluble proteins accounting for almost 50% of total protein in the mammalian transparent eye lens, also present in a variety of other tissues. Crystallin families beta and gamma share homology with each other but not the α family or the small heat shock protein (sHsp) family, while Alpha-crystallins, induced by heat in a variety of organisms, belong to the sHsp family. The alpha-crystallins possess structural and functional similarities and share sequence homology with Hsp25/273. Most sHsps exhibit four common structural and functional features: molecular weight between 12 and 43kDa; the formation of large oligomeric complexes composed of alphaA-crystallin, alphaB-crystallin and Hsp25/27; a moderately conserved alpha-crystallin domain in the central region of the protein; and molecular chaperone activity. The alpha-crystallin domain bounded by variable N-terminal and C-terminal extensions contains approximately 90 residues and participates in oligomer assembly. Oligomers, potentially 800kDa or more, exhibit dynamic subunit exchanges and organizational plasticity, which may promote functional diversity. Phosphorylation of serine residues occurs in response to stress during development, typically decreasing oligomer size. Chaperone activity requires oligomerization (which, in turn, modulates the chaperone activity) and is confined to binding unfolded intermediates to prevent irreversible aggregation, even though productive release and refolding of denatured proteins requires close cooperation with other chaperones. Other proposed functions include a role in membrane stabilization and modulation of intermediate filament organization during physiological stress and neurodegenerative disease.

Applications
IP, WB

Format
Whole rabbit serum

Host Species
rabbit

Immunogen
Synthetic peptide derived from sequence near the carboxy-terminus of human alpha A-Crystallin, conjugated to KLH

Purification Method
Antiserum

Species Reactivity
B

For research use only, not for diagnostic or therapeutic use.

Documentation
TechSpec

Product Manual

Product Name Catalog # Manual
Crystallin, AlphaA Polyclonal Antibody SPA-221F  
Crystallin, AlphaA Polyclonal Antibody SPA-221D  

Related Products

Product Name Catalog#
Crystallin, AlphaA Native Protein
 		SPP-226
Crystallin, AlphaA/AlphaB Polyclonal Antibody
 		SPA-224

Citations
Launch Citation Database

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