Hsp90 alpha ELISA Kit

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
Hsp90 alpha ELISA Kit 1x96 well  EKS-895 $670.00
Background
The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

Table of Abbreviations

Applications
EIA

Format
ELISA

Range
0 - 4 ng/mL

Sample Size
100µL/well

Product Image(s): click to enlarge

Sample Types
cell lysates, tissue extracts, serum

Samples Per Kit
32-40 samples in duplicate per 96 well kit

Sensitivity
50 pg/mL

Species Reactivity
H

Time To Answer
< 3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
MSDS
Kit Insert

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Citations
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