TNF-alpha (rat) EIA Kit

Brand: Assay Designs

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Description	Size	Catalog#	Price	Quantity
TNF-alpha (rat) EIA Kit	1x96 well	900-086A	$495.00

Background

Tumor necrosis factor alpha (TNFalpha), also known as cachectin, is a 17.5 kDa 157 amino acid member of the TNF superfamily of cytokines that is a potent lymphoid factor with effects on a wide range of target cells. Active TNFalpha is produced in both soluble and membrane-anchored trimers by macrophages, NK cells, and T- and B-lymphocytes. TNF exerts proinflammatory signals via binding and inducing trimerization of TNF-receptor1 (TNF-R1) expressed on most normal and transformed cells, or to TNF-receptor 2 (TNF-R2), expressed on endothelial and most immune cells. TNF signaling regulates hematopeiesis, differentation, endothelial cell activation, apoptosis, lipid metabolism, tumor progression, and immune suveillance, and dysregulation of TNF or its receptors is implicated in numerous disease states including cancer, osteoporosis, autoimmune disease, diabetes, and atherosclerosis.

Table of Abbreviations

Applications
EIA

Features

Sensitive ELISA for the quantification of TNF alpha in rat culture supernates
Fast results in just 3 hours
Validated in culture supernates +/- 10% FBS

Range
31.3 - 2,000 pg/mL

Sample Size
100 無

Cross Reactivities
rat TNF-alpha 100%
No significant reactivity was observed with human, mouse, or porcine TNF alpha or human TNF beta

Sample Types
culture supernates

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
12.0 pg/mL in Assay Buffer; 26.7 pg/mL in culture media supplemented with 10% FBS

Species Reactivity
R

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Assay Layout
Kit Insert
MSDS
Technical Bulletin

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Stop Solution 2, 10 mL	80-0377
TMB Substrate, 10 mL	80-0350
TNF Receptor 1 (TNF-R1) Polyclonal Antibody	CSA-815E
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Citations
Launch Citation Database

Ordering Info
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TNF-alpha (rat) EIA Kit