Catalase Fluorometric Detection Kit

Product Comments
Assay Designs' Catalase Fluorometric Detection Kit is a sensitive fluorescent assay to detect catalase activity by measuring the amount of substrate (hydrogen peroxide) remaining after sample addition. This kit provides a simple homogenous assay that is adaptable to kinetic and High Throughput applications.

Applications
Activity

Format
Fluorescence

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Features

• Fast results in just ~1 hour
• High Sensitivity Fluorescent Assay
• Suitable for High Throughput Screening
• Monitor multiple time points to follow kinetics
• Simple no-wash homogenous assay
• Positive Control Provided

Range
0 - 4 U/mL

Sample Types
cell lysates

Significance and Uses
Catalase is an antioxidant enzyme that catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Catalase is ubiquitously expressed in mammalian and non-mammalian aerobic cells containing the cytochrome system and is a protein tetramer of four ferrihemoprotein groups per molecule. The enzyme has been isolated from various sources, including bacteria and plant cells. Catalase activity varies greatly from tissue to tissue. Highest activity is seen in liver and kidney, while lowest activity is seen in connective tissue. The presence of catalase in the peroxisomes of mammalian cells helps to alleviate oxidative damage by catalyzing the metabolism of the peroxide substrate. The production of H2O2, a highly reactive byproduct of mammalian metabolism, can be deleterious to cells and is an end product result of various oxidase and superoxide dismutase reactions. Accumulation of H2O2 can result in cellular damage through oxidation of proteins, DNA, and lipids thus resulting in cell death and mutagenisis. The role of H2O2 in oxidative stress related pathologies (e.g., inflammation, cancer, diabetes, cardiovascular disease, anemia, Parkinson¿s disease, Alzheimer¿s disease) has been widely studied.

Species Reactivity
Species Independent

Time to Answer
~ 1 hour

For research use only, not for diagnostic or therapeutic use.