COMET SCGE Assay

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Description Size Catalog# Price Quantity  
COMET SCGE Assay 50 tests  900-166 $225.00
Background
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage. Assay Designs? COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic ?comet? shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA. The Stressgen® DNA Damage Kit is a competitive ELISA for the detection and quantification of 8-hydroxy-2'-deoxyguanosine (8-OHdG), an oxidized nucleoside of DNA that is excised from damaged DNA and present in urine and serum. 8-OHdG is a frequently used biomarker of oxidative DNA damage and oxidative stress, and may serve as a risk factor for cancer, atherosclerosis and diabetes.

Table of Abbreviations

Applications
DNA Damage/Repair and Apoptosis detection

Format
Electrophoresis Based Assay

Features

  • Ready-to-use Comet Slides allow direct application of sample without pretreatment
  • Shorter assay time allows for higher throughput sample analysis
  • Hydrophobic barrier allows sample treatment with DNA repair enzymes
  • Unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide

Sample Types
cells and tissue

Samples Per Kit
50 tests

Species Reactivity
Species Independent

Time To Answer
< 4 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert
MSDS

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Citations
Launch Citation Database

Ordering Info
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