HO-2 Recombinant Human Protein

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Description Size Catalog# Price Quantity  
HO-2 Recombinant Human Protein  50 µg SPP-550D $175.00  
HO-2 Recombinant Human Protein  200 µg SPP-550E $425.00  
Background
Heme oxygenase-2 (HO-2), the constitutive isoform of heme oxygenase, catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carb on monoxide, iron and biliverdin (immediately reduced to bilirubin). These products of the HO reaction render important physiological effects. Carbon monoxide becomes a potent vasodilator, biliverdin and its product bilirubin function as potent antioxidants, and ¿free¿ iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT -1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein-1 (IRP-1) and its binding to iron regulatory elements (IREs) in the 5¿- or 3¿-UTRs of the mRNAs. To date, researchers have identified heme oxygenase isoforms HO -1, HO-2 and HO-3. The mRNA and activity of HO -1/Hsp32, a ubiquitous major heat shock/stress response protein, can be increased several-fold by heme, other metalloporphyrins, transition metals and stimuli that induce cellular stress. In contrast to HO-1, HO-2 is highly concentrated in neurons and testes. Researchers identified multiple HO -2 transcripts which differ in size and use 3 different 5¿ untrans lated regions (referred to as rHO-2, rHO-2-1 and rHO-2-2) and 2 poly(A) signals, also characterizing a functional glucocorticoid response element (GRE) in the promoter region of rHO -2. In the adult rat testis, data shows developmentally regulated expression of two transcripts for HO -2 of ~2.1kb and ~1.45kb not present in the brain, kidney, thymus, heart, spleen, liver or in prepubertal 14 day old rat testis. Both transcripts exclusively use rHO-2 and contain all coding region exons present in the ~1.3kb a nd ~1.9kb transcripts and common to all other organs, including the adult and prepubertal rat testis. This data suggests that glucocorticoids control HO -2 levels in the testis, and that developmental and tissue-specific factors determine generation of tran scripts unique to the organ. The apparent exclusive use of rHO-2 by the mature testis indicates HO-2 may play a role in male reproduction.

Table of Abbreviations

Abbreviated Name
HO-2 Recomb Human Protein

Expression System
Recombinant human HO-2 was cloned from human placenta and expressed in E. coli.

Species Reactivity
not applicable

Applications
WB Control, IHC Control

Purification
>90% pure as determined by SDS-PAGE analysis

Source
Human

For research use only, not for diagnostic or therapeutic use.

Documentation
Spec Sheet

Product Manual

Product Name Catalog # Manual
HO-2 Recombinant Human Protein SPP-550D  
HO-2 Recombinant Human Protein SPP-550E  

Related Products

Product Name Catalog#
Cytochrome P450 Reductase Polyclonal Antibody
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HO-2 Polyclonal Antibody
 		OSA-200
HO-2 Polyclonal Antibody
 		SPA-897
HO-2 Recombinant Rat Protein
 		OSP-250
Human Liver Microsome Extract
 		LYT-HM100F
Mouse Liver Microsome Extract
 		LYT-MM100F
Rat Liver Microsome Extract
 		LYT-RM100F

Citations
Launch Citation Database

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