DNA Damage ELISA Kit

Brand: Stressgen Print Page
Description Size Catalog# Price Quantity  
DNA Damage ELISA Kit 1x96 well  EKS-350 $720.00
Background
Stressgen's DNA Damage ELISA (enzyme-linked immunosorbent assay) is a fast and sensitive competitive immunoassay for the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in urine and serum samples. 8-OHdG has become a frequently used biomarker of oxidative DNA damage and oxidative stress. Measurement of urinary 8-OHdG may be useful as an indicator of oxidative damage. The DNA Damage ELISA uses an 8-OHdG monoclonal antibody to bind, in a competitive manner, 8-OHdG in the sample, standard or pre-bound to the wells of the 96-well immunoassay plate. Anti-8-OHdG bound to 8-OHdG in the sample or standard are washed away while those captured by the immobilized 8-OHdG are detected with a secondary antibody: HRP conjugate. The assay is developed with tetramethylbenzidine substrate and the absorbance is measured in a microplate reader at 450nm. The intensity of the yellow color is inversely proportional to the concentration of 8-OHdG.

Product Comments
The DNA Damage ELISA is a rapid and sensitive competitive immunoassay for the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in urine, serum, and saliva samples. The assay is easy to use and has a simple, quick protocol. The kit contains all the necessary reagents for fast, consistent results, including ready-to-use pre-coated 96-well plate, 8-OHdG standard, diluents, buffers, HRP conjugate, TMB substrate, stop solution, and instruction manual. An Excel spreadsheet for easy calculation of results is available under the 'documentation' tab above. Please see kit insert for details.

Applications
EIA

Format
ELISA

Range
0.94 - 60 ng/mL

Sample Size
50µL/well

Sample Types
urine, serum, and saliva

Samples Per Kit
39 samples in duplicate per 96 well kit

Sensitivity
0.59 ng/mL

Species Reactivity
Species Independent

Time To Answer
< 2.5 hours

For research use only, not for diagnostic or therapeutic use.

Ordering Info
Click here to learn more about online ordering
Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134