HO-1 (Rat) ELISA Kit

Brand: Stressgen

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Description	Size	Catalog#	Price	Quantity
HO-1 (Rat) ELISA Kit	1x96 well	EKS-810A	$585.00

Background

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

Table of Abbreviations

Features

Thoroughly validated in serum, plasma, cell lysates, and tissue extracts
Highly sensitive quantification of HO-1 surpass semi-quantitative Western blot results
Accurate and reproducible results in less than 3 hours

Product Image(s): click to enlarge

Applications
EIA

Cross Reactivities
<0.02%: rat HO-2 / mouse HO-1 / human HO-1

Format
ELISA

Range
0.195 - 12.5 ng/mL

Sample Size
100無/well

Sample Types
cell lysates, tissue extracts, serum, and plasma

Samples Per Kit
39 samples in duplicate per 96 well kit

Sensitivity
0.036 ng/mL

Species Reactivity
R

Time To Answer
< 3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
Kit Insert

Related Products

Product Name	Catalog#
8-iso-PGF2alpha (direct) EIA Kit	900-091
8-iso-PGF2alpha EIA Kit	900-010
HO-1 (Hsp32) Monoclonal Antibody (HO-1-1)	OSA-110
HO-1 (Hsp32) Monoclonal Antibody (HO-1-2 )	OSA-111
HO-1 (Hsp32) Monoclonal Antibody (HO-1-2 ), FITC Conjugate	OSA-111FI
HO-1 (Hsp32) Monoclonal Antibody (HO-1-2), PE Conjugate	OSA-111PE
HO-1 (Hsp32) Polyclonal Antibody	SPA-895
HO-1 (Hsp32) Polyclonal Antibody	SPA-896
HO-1 (Hsp32) Polyclonal Antibody	SPA-894
HO-1 (Human) ELISA Kit	EKS-800
Heme Oxygenase-1 Monoclonal Antibody (HO-1-2), DyLight(TM) 488 Conjuga	OSA-111-488
Heme Oxygenase-1 Monoclonal Antibody (HO-1-2), DyLight(TM) 488 Conjugate	OSA-111-488
Rat Liver Microsome Extract	LYT-RM100F
Stop Solution 2	80-0377
TMB Substrate	80-0350
Wash Buffer Concentrate	80-1287

Citations
Launch Citation Database

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HO-1 (Rat) ELISA Kit