Cytochrome c (human) EIA Kit

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Description Size Catalog# Price Quantity  
Cytochrome c (human) EIA Kit 1x96 well  900-141 $525.00
Background
Cytochrome c, which is a critical component of the electron transport chain, is a component of the functional apoptosome. Upon release from the mitochondria, cytochrome c binds to the adaptor proteins Apaf-1 and procaspase-9 to form a cytosolic apoptosome complex. This event results in activation of procaspase-9 and its subsequent cleavage and activation of caspase-3 leading to the degradation of a series of cellular substrates and subsequently cell death.

Table of Abbreviations

Applications
EIA

Format
ELISA

Features

  • Validated in both cytosolic and mitochondrial fractions of cell lysates
  • Most sensitive Cytochrome c immunoassay on the market

Range
28.13 - 900 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
human Cytochrome c 100%
rat Cytochrome c 6.41%
equine Cytochrome c 0.92%
canine Cytochrome c 0.62%
bovine Cytochrome c 0.61%
<0.03%: Bax, Bcl-2, Bcl-XL, Caspase-9, cIAP-1, Survivin

Sample Types
RIPA Cell Lysis Buffer 2,Subcellular pellet lysed in RIPA,Digitonin Cell Permeabilization Buffer,Cystolic fraction lysed in Digitonin

Samples Per Kit
40 in duplicate per 96 well kit

Sensitivity
6.03 pg/mL

Species Reactivity
H

Time To Answer
3 hours, 15 minutes

For research use only, not for diagnostic or therapeutic use.

Ordering Info
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