ERK1/2 (phospho) EIA Kit

Applications
EIA

Format
ELISA

Features

• Results in just 3 hours
• Much easier than Western blotting

Range
62.5 - 2,000 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
phospho ERK 100%
<0.01%: non-phosphorylated ERK / phospho p38 / non-phosphorylated p38 / phospho JNK / non-phosphorylated JNK / phospho AKT / non-phosphorylated AKT

Sample Types
RIPA cell lysis buffer

Samples Per Kit
40 in duplicate per 96 well kit

Sensitivity
38.3 pg/mL

Significance and Uses
The mitogen-activated protein kinase (MAPK) pathway exists in all eukaryotes. It consists of several subgroups including ERK 1/2 (extracellular signal-regulated kinase), JNK and p38 kinases. These kinases regulate and control fundamental cellular processes including proliferation, apoptosis, survival and differentiation. They are activated by diverse stimuli including cytokines, growth factors, irradiation, changes in osmolarity, heat and sheer stress. MAP kinases are characterized by their requirement for dual phosphorylation for full activation. ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif . The literature contains numerous and extensive reviews on MAP kinase.

Species Reactivity
H, M, R

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.