cyclic GMP EIA Kit

Brand: Assay Designs

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Description	Size	Catalog#	Price	Quantity
cyclic GMP EIA Kit	1x96 well	900-013	$320.00
cyclic GMP EIA Kit	5x96 well	901-013	$1,275.00

Background

Guanosine 3', 5'-cyclic monophosphate (cyclic GMP; cGMP) was identified in 1963. It has been shown to be present at levels typically 10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. It is involved in a number of important biological reactions. Some hormones, such as acetylcholine, insulin and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate and nitric oxide (NO) also stimulate cGMP levels. Peptides, such as, atrial natriuretic peptide (ANP) that relax smooth muscle also increase cGMP concentrations. cGMP has been confirmed as a second messenger for ANP. NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells.

Applications
EIA

Format
ELISA

Product Image(s): click to enlarge

Features

Results in just 3 hours
Convenient 4蚓 storage
Measure cGMP in biological fluids

Range
(non-acetylated) 0.16 - 500pmol/mL; (acetylated) 0.16 - 100pmol/mL

Sample Size
100 無

Cross Reactivities
cyclic GMP 100%
GMP <0.001%
GTP <0.001%
cyclic AMP <0.001%
AMP <0.001%
ATP <0.001%
cyclic UMP <0.001%
CTP <0.001%

Sample Types
culture supernates, saliva, serum, plasma, urine

Samples Per Kit
(non-acetylated) 38 in duplicate per 96 well kit; (acetylated) 39 in duplicate per 96 well kit

Sensitivity
(non-acetylated) 0.37 pmol/mL; (acetylated) 0.088 pmol/mL

Significance and Uses
Assay Designs' Regulart cyclic GMP (cGMP) kit is designed for the quantitative determination of cGMP in culture supernates, saliva, and serum. An optional acetylated protocol is available to increase sensitivity when needed.

Species Reactivity
Species Independent

Time To Answer
3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name	Catalog #	Manual
cyclic GMP EIA Kit	900-013	Kit Insert MSDS
cyclic GMP EIA Kit	901-013	Kit Insert MSDS

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Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134

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