Cytochrome c (human) EIA Kit

Applications
EIA

Format
ELISA

Features

• Validated in both cytosolic and mitochondrial fractions of cell lysates
• Most sensitive Cytochrome c immunoassay on the market

Range
28.13 - 900 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
human Cytochrome c 100%
rat Cytochrome c 6.41%
equine Cytochrome c 0.92%
canine Cytochrome c 0.62%
bovine Cytochrome c 0.61%
<0.03%: Bax, Bcl-2, Bcl-XL, Caspase-9, cIAP-1, Survivin

Sample Types
RIPA Cell Lysis Buffer 2,Subcellular pellet lysed in RIPA,Digitonin Cell Permeabilization Buffer,Cystolic fraction lysed in Digitonin

Samples Per Kit
40 in duplicate per 96 well kit

Sensitivity
6.03 pg/mL

Significance and Uses
Cytochrome c, ~13 kDa and 105 amino acids long, is a nuclear-encoded component of the mitochondrial respiratory chain that is imported as an apoenzyme into mitochondria, where it is converted to the mature form by the addition of a heme group. It catalyzes electron transfer between complexes III and IV of the respiratory chain, moving within the planar surface of the inner mitochondrial membrane. In addition to Cytochrome c's role in oxidative phosphorylation, the release of Cytochrome c from the mitochondrial intermembrane space to the cytosol results in nuclear apoptosis. Binding of APAF1 to Cytochrome c allows APAF1 to form a ternary complex with and activate the initiator Procaspase-9 in the presence of dATP. Active Caspase-9 then triggers downstream effector caspases, beginning the death cascade apoptosis. Recent studies have demonstrated that Cytochrome c directly microinjected in the cytoplasms of a variety of cell types is capable of initiating apoptosis on its own, while APAF-1 is needed as a cofactor in cell free extracts thus emphasizing Cytochrome c's crucial role in apoptosis. In contrast, examples of apoptosis without Cytochrome c release indicate that this event may not be necessary in all apoptotic pathways.

Species Reactivity
H

Time To Answer
3 hours, 15 minutes

For research use only, not for diagnostic or therapeutic use.