12(S)-HETE EIA Kit

Brand: Assay Designs

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Description	Size	Catalog#	Price	Quantity
12(S)-HETE EIA Kit	1x96 well	900-050	$360.00
12(S)-HETE EIA Kit	5x96 well	901-050	$1,440.00

Background

12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE has been shown to be chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. Evidence also suggests that 12(S)-HETE is involved in suppressing renin production, stimulating insulin secretion by pancreatic tissue, inducing endothelial cell retraction and tumor cell adhesion. It also regulates platelet aggregation and induces heat shock proteins in human leukocyctes.

Applications
EIA

Format
ELISA

Features

Only 12(S)-HETE immunoassay on the market
Much easier than GC/MS measurement

Range
195 - 50,000 pg/mL

Sample Size
100 無

Product Image(s): click to enlarge

Cross Reactivities
12(S)-HETE 100%
12(R)-HETE 2.50%
15-HETE 0.30%
5(S)-HETE 0.20%
0.10%: 8,15-diHETE / 5,15-diHETE / PGE2 / PGF2alpha / PGD2 / 6-keto-PGF1alpha / TXB2 / Arachidonic Acid / LTB4 / LTC4 / LTD4 / LTE4
<0.1%: 8-HETE / 9-HETE / 11-HETE

Sample Types
culture media, plasma

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
146 pg/mL

Species Reactivity
Species Independent

Time To Answer
5 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name	Catalog #	Manual
12(S)-HETE EIA Kit	900-050	Kit Insert MSDS
12(S)-HETE EIA Kit	901-050	Kit Insert MSDS

Related Products

Product Name	Catalog#
12(L)-HETE Polyclonal Antibody	905-053
12-HETE Polyclonal Antibody	905-032
15(S)-HETE EIA Kit	900-051
Cysteinyl Leukotriene EIA Kit	900-070
LTB4 EIA Kit	900-068
PGE2 EIA Kit	900-001
PGF2alpha EIA Kit	900-069
TXB2 EIA Kit	900-002

Citations
Launch Citation Database

Ordering Info
Click here to learn more about online ordering
Order by phone: 734-668-6113 or 877-423-4548
Order by e-mail: orders@assaydesigns.com
Order by fax: 734-669-0134

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12(S)-HETE EIA Kit