Urinary Prostacyclin EIA Kit

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Description Size Catalog# Price Quantity  
Urinary Prostacyclin EIA Kit  1x96 well 900-025 $345.00  
Urinary Prostacyclin EIA Kit  5x96 well 901-025 $1,395.00  
Background
Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6-keto-PGF1alpha and 2,3-dinor-6-keto-PGF1alpha. 6-keto-PGF1alpha is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6-keto-PGF1alpha. The conversion of PGI2 into 6-keto-PGF1alpha and 2,3-dinor-6-keto-PGF1alpha is shown below.

Table of Abbreviations

Applications
EIA

Format
ELISA

Features

  • Measure both major PGI2 breakdown products (2,3-dinor and 6-keto-PGF1a) in one assay
  • Excellent sensitivity
  • Results in <3 hours

Range
7.81 - 2,000 pg/mL

Sample Size
100 µL

Product Image(s): click to enlarge

Cross Reactivities
2,3-dinor-6-keto-PGF1alpha 100%
6-keto-PGF1alpha 89.1%
PGF1alpha 13.2%
PGF2alpha 4.99%
13,14 dihydro-PGF1alpha 2.55%
15-keto-PGF2alpha 0.32%
PGE1 0.08%
PGD2 0.03%
6,15-diketo-13,14 dihydro-PGF1alpha 0.03%
PGB1 0.01%
PGE2 0.01%
PGA2 0.01%
TXB2 0.01%

Sample Types
culture media, urine

Samples Per Kit
39 in duplicate per 96 well kit

Sensitivity
6.58 pg/mL

Species Reactivity
Species Independent

Time To Answer
< 3 hours

For research use only, not for diagnostic or therapeutic use.

Documentation
AssayLayout

Product Manual

Product Name Catalog # Manual
Urinary Prostacyclin EIA Kit 900-025   Kit Insert    MSDS   
Urinary Prostacyclin EIA Kit 901-025   Kit Insert    MSDS   

Related Products

Product Name Catalog#
11-dehydro-TXB2 EIA Kit
 		900-092
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2,3-dinor-6-keto-Prostaglandin F1alpha Polyclonal Antibody
 		905-004
6-keto PGF1alpha EIA Kit
 		900-004
6-keto PGF1alpha EIA Kit
 		901-004
6-keto-PGF1 alpha Affinity Resin **
 		RES-100
8-iso-PGF2alpha EIA Kit
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COX Activity Kit
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Citations
Launch Citation Database

Ordering Info
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